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Enzyme Lab Answers: A Comprehensive Guide to Understanding Your Results
Are you staring at your enzyme lab report, feeling utterly bewildered? Understanding enzyme activity can be challenging, but it doesn't have to be. This comprehensive guide provides detailed explanations and interpretations to help you make sense of your enzyme lab answers, regardless of the specific experiment conducted. We'll dissect common enzyme experiments, explain the key concepts behind the results, and offer tips for troubleshooting any unexpected findings. Get ready to unlock the secrets of your enzyme lab data!
Understanding Enzyme Activity: The Basics
Before diving into specific lab results, let's review the fundamental principles of enzyme activity. Enzymes are biological catalysts, proteins that speed up chemical reactions without being consumed themselves. Their activity is influenced by various factors, including:
Substrate Concentration: Increasing substrate concentration generally increases reaction rate until a saturation point is reached.
Temperature: Enzymes have optimal temperature ranges; deviations can lead to decreased or even complete loss of activity.
pH: Similar to temperature, enzymes function best within a specific pH range.
Enzyme Concentration: Higher enzyme concentration usually leads to a faster reaction rate.
Inhibitors: Certain molecules can bind to enzymes and reduce their activity.
Understanding these factors is crucial for interpreting your lab results.
Common Enzyme Lab Experiments and Their Interpretation
Many enzyme labs focus on demonstrating these factors. Let's explore some common experiments and how to interpret the results:
#### 1. Effect of Substrate Concentration on Enzyme Activity:
This experiment typically involves measuring reaction rate at different substrate concentrations. Your data should show an initial increase in reaction rate with increasing substrate concentration, eventually plateauing as the enzyme becomes saturated. Analyzing your data: Look for the point of saturation – this indicates the maximum reaction rate achievable under the given conditions. Any deviations from this expected pattern may indicate experimental error or the presence of inhibitors.
#### 2. Effect of Temperature on Enzyme Activity:
This experiment tests enzyme activity at different temperatures. You should observe an optimal temperature where activity is highest. At lower temperatures, activity is reduced due to slower molecular movement. At higher temperatures, activity decreases and potentially ceases due to enzyme denaturation (loss of protein structure). Analyzing your data: Identify the optimal temperature and note the temperature ranges where activity significantly decreases.
#### 3. Effect of pH on Enzyme Activity:
Similar to the temperature experiment, this explores enzyme activity across a range of pH values. Each enzyme has an optimal pH; deviations lead to decreased activity. Extreme pH changes can denature the enzyme. Analyzing your data: Identify the optimal pH for the enzyme and the range where activity is significantly affected.
#### 4. Effect of Enzyme Concentration on Enzyme Activity:
This experiment examines the relationship between enzyme concentration and reaction rate. Generally, increasing enzyme concentration leads to a faster reaction rate, assuming sufficient substrate is present. Analyzing your data: Look for a linear relationship between enzyme concentration and reaction rate. Deviations might indicate limitations in substrate availability or the presence of inhibitors.
#### 5. Enzyme Inhibition Experiments:
These experiments involve introducing inhibitors (competitive or non-competitive) to observe their effect on enzyme activity. Competitive inhibitors compete with the substrate for the enzyme's active site, while non-competitive inhibitors bind elsewhere, altering the enzyme's shape. Analyzing your data: Compare the reaction rates in the presence and absence of inhibitors. Competitive inhibitors show reduced reaction rates that can be overcome with high substrate concentrations, whereas non-competitive inhibitors show reduced rates that are not overcome by increased substrate.
Troubleshooting Your Enzyme Lab Answers
Unexpected results are common in enzyme experiments. Here are some potential sources of error:
Improperly prepared solutions: Ensure accurate concentrations and proper mixing.
Contamination: Contaminants can affect enzyme activity.
Incorrect use of equipment: Follow instructions carefully for spectrophotometers, timers, etc.
Temperature fluctuations: Maintain consistent temperature throughout the experiment.
If your results deviate significantly from expectations, carefully review your methodology and consider repeating the experiment with improved controls.
Conclusion
Understanding your enzyme lab answers requires a solid grasp of enzyme kinetics and experimental design. By carefully analyzing your data in light of the factors affecting enzyme activity, you can gain valuable insights into the behavior of these crucial biological molecules. Remember to meticulously record your data, account for potential errors, and utilize resources to help you interpret your findings. With practice and attention to detail, interpreting enzyme lab results will become significantly easier.
Frequently Asked Questions (FAQs)
1. What units are typically used to measure enzyme activity? Enzyme activity is often expressed in units of micromoles (µmol) of substrate converted per minute per milligram (mg) of enzyme (µmol/min/mg). However, other units may be used depending on the specific experiment and assay.
2. How can I improve the accuracy of my enzyme lab results? Precise measurements, careful controls, and repetition of experiments are crucial for improving accuracy. Also, ensure proper calibration of any equipment used.
3. What are some common errors in enzyme lab experiments? Common errors include inaccurate measurements, contamination of samples, improper temperature control, and neglecting to account for blank readings in spectrophotometric assays.
4. My enzyme activity is significantly lower than expected. What could be the cause? Several factors could be at play, including enzyme denaturation (due to temperature or pH extremes), the presence of inhibitors, insufficient substrate, or experimental error.
5. Where can I find more information on enzyme kinetics? Numerous textbooks and online resources cover enzyme kinetics in detail. Search for "enzyme kinetics" in your preferred academic database or online search engine.
| enzyme lab answers: Laboratory Exercises in Microbiology Robert A. Pollack, Lorraine Findlay, Walter Mondschein, R. Ronald Modesto, 2018-07-11 The Laboratory Exercises in Microbiology, 5e by Pollack, et al. presents exercises and experiments covered in a 1 or 2-semester undergraduate microbiology laboratory course for allied health students. The labs are introduced in a clear and concise manner, while maintaining a student-friendly tone. The manual contains a variety of interactive activities and experiments that teach students the basic concepts of microbiology. The 5th edition contains new and updated labs that cover a wide array of topics, including identification of microbes, microbial biochemistry, medical microbiology, food microbiology, and environmental microbiology. |
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| enzyme lab answers: Cranial Creations in Physical Science Charles R. Downing, Candace J. Aguirre, 1994 Lively assignments include: Energy: The Choice is Yours Rain, Rain, Go Away My Fossil's Older Than Your Fossil Spend Some Time in the O Zone Death of the Sun An Interview with Galileo A Trip to My Favorite Planet That Really Burns Me Up Faster Than a Speeding...Snail? Funnels of Fun |
| enzyme lab answers: Teacher's Wraparound Edition: Twe Biology Everyday Experience Albert Kaskel, 1994-04-19 |
Lee 1 Jessica Lee AP Biology 23 October 2013 - Region 18
This lab consisted of four different experiments that were carried out to determine the effects of substrate concentration, enzyme concentration, and inhibitor, and temperature on enzyme …
Lactose Lab Answer Key - agclassroom.org
Nov 16, 2015 · Lactose Lab Answer Key. 1. Why is it important to have a “control” in your experiment? Having a control in an experiment gives more accurate test results. In this lab it is …
Lactase enzyme lab - MRS. OSBORNE'S CLASS
This lab will examine the specificity of an enzyme (lactase) to a specific substrate (lactose). Students will observe the actions of the enzyme and how shape is important to enzyme reactions.
Lab: Enzyme Catalysis (Modified AP Lab 2A) Name - KABT
INTRODUCTION: In general, enzymes are proteins produced by living cells; they act as catalysts in biochemical reactions. A catalyst affects the rate of a chemical reaction. One consequence …
Enzymes: Practice Questions #1
Enzymes: Practice Questions #1. Compound X increases the rate of the reaction below. Compound X is most likely. an enzyme. a lipid molecule. an indicator. an ADP molecule. The …
TOOTHPICKASE - Enzyme Simulation - West Linn-Wilsonville …
This lab will serve as a pre-lab activity for AP Biology Lab #2 on enzyme catalysis. In this activity you (or rather, your hands) will become the enzyme called “toothpickase”.
Investigation 13: Enzyme Activity Notes From the teacher Day …
Investigation 13: Enzyme Activity Notes From the teacher Day 1: Before class: Read Entire Lab and Complete Pre Lab. Pre-Lab: 1. What is the difference between catalytic and anabolic …
Toothpick-ASE Lab: Introduction to Enzymes (SB1b, SB1c)
Toothpick-ASE Lab: Introduction to Enzymes (SB1b, SB1c) Enzymes are used in all metabolic reactions (catabolic and anabolic) to control the rate of reactions and decrease the amount of …
BACKGROUND - College Board
The main enzyme for this lab, peroxidase, is found in many different forms, with optimum pHs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to …
The Need for Speed—A Look at Enzyme Activity - Chino …
The Need for Speed—A Look at Enzyme Activity. Introduction: Enzymes are proteins that are catalysts. This means they speed up chemical reactions in living organisms, but they aren’t …
AP Biology Lab: Enzyme Reaction Rates Using Toothpickase
AP Biology Name Enzyme Catalysis Lab - Livingston Public …
Directions for lab questions and information you need to know for the quiz on the Enzyme Lab You do NOT have to write out any answers for questions 1-2 or 6 but you should know the …
Restriction Enzyme Cleavage of DNA and Electrophoresis (AP …
Bacillus globigii Restriction enzymes are endonucleases that catalyze cleavage of phosphodiester bonds within both strands of DNA. They require Mg+2 Bacillus amyloliquefaciens H for activity …
Online Enzyme Lab Simulation - BIOLOGY4IBDP
AP Biology Lab Manual - Weebly
Experiment 10 – Enzymes - Laney College
Jan 10, 2012 · All biochemical reactions are catalyzed by enzymes. Since enzymes are proteins, they can be denatured in a variety of ways, so they are most active under mild conditions. …
Lab 05 - Enzymes & Diffusion - PCC
Pre-lab Homework for Lab 5: Enzymes & Diffusion. After reading over the lab and the enzyme, diffusion and osmosis topics from your textbook, answer these questions to be turned in at the …
1-6 Virtual Enzyme Lab - Grace's Biology Blog
Enzymes: What’s in your spit? - Stanford University
Students know the individual functions and sites of secretion of digestive enzymes (amylases, proteases, nucleases, lipases), stomach acid, and bile salts. Prerequisites: • Good for all …
TOOTHPICKASE: MODELLING ENZYME ACTIVITY - Mrs.
Liver Enzyme Lab - Brown Biology
Microsoft Word - Liver Enzyme Lab.doc Author: Kelly Brown Created Date: 9/10/2012 2:17:57 AM ...
Lab 3 Enzymes Pre Lab 3 Test Questions - De Anza College
BIO10 Lab 3 Enzymes and pH 28 Lab 3: ENZYMES Objectives: • Discuss the function and role of enzymes in living organisms. • Identify the substrate (reactant), enzyme and product of a chemical reaction. • Differentiate between exergonic and endergonic reactions. • List 5 things that influence enzyme activity and
Bio 6 – Restriction Enzyme Mapping Lab - Los Angeles …
to the Restriction Enzyme Digestion Lab to load, run and stain your gel (NOTE: You should also be able to prepare and run your gel by following your procedure for this lab in your lab notebook). Exercise 2 – Agarose gel electrophoresis of restriction digests 1. Pour a 60 ml 1% agarose/1X TAE gel keeping in mind that you will need at least 9 ...
Toothpickase Lab Answers - exmon01.external.cshl.edu
Toothpickase Lab Answers Marcel A. Müller AP Biology Lab: Enzyme Reaction Rates Using Toothpickase In this activity you will become the enzyme called ... Enzyme Lab: Toothpickase Enzyme Lab: “Toothpickase” (“Toothpickase” is a DIGESTIVE ENZYME. It breaks down toothpicks into two units.) About Enzymes Chemical reactions make life ...
The Enzymatic Activity of Lactase - Massachusetts Farm to …
Mar 7, 2023 · Students: Lab Procedure 1. Label the test tubes with the following labels: A) skim milk and enzyme solution. B) skim milk and distilled water. C) sucrose solution and enzyme solution. D) sucrose solution and water. 2. In test tube A, add 2 mL of skim milk and 1 mL of enzyme solution. Time for 2 minutes and test for glucose with the glucose test ...
Lab #10 - Toothpickase, An Introduction to Enzymes
you will play the part of an enzyme. In this lab, the toothpicks represent the substrate, you are the enzymes and your fingers represent the enzyme's active site. The "toothpickase" enzyme catalyzes the breakage of the toothpicks into two pieces. This experiment will demonstrate how enzymatic rate of reactions can be
Factors Affecting Enzyme Activity - Purdue University
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Biofuel Enzyme Reactions Kit for AP Biology: - Bio-Rad
so that more substrate and enzyme will be able to react. However, in an enzymatic reaction, too much heat is a bad thing. You may recall from studying about proteins that the noncovalent interactions within the protein, such as hydrogen and ionic bonds, can break at high temperatures. This will change the shape of the enzyme. If the enzyme changes
Enzyme Lab: Toothpickase
Enzyme Lab: “Toothpickase” (“Toothpickase” is a DIGESTIVE ENZYME. It breaks down toothpicks into two units.) About Enzymes Chemical reactions make life possible. In each individual cell of a human there are many chemical reactions taking place. These chemical reactions involve the breaking and reforming of chemical bonds between
What effect enzyme activity? Toothpickase - University of …
have another person in your group act as the enzyme or toothpick-ase. The person acting as the enzyme is to break toothpicks without looking at the bowl and all of its products (broken toothpicks). All broken toothpicks must remain in the bowl along with the unbroken toothpicks, & you cannot re-break a broken toothpick!.
Cellular Energetics
§ AP Biology Lab Manual > Enzyme Lab § Classroom Resources > Visualizing Information TOPIC 3.3 Environmental Impacts on Enzyme Function Cellular Energetics UNIT3 continued on next page 70 | Course and Exam DescriptionCourse Framework V.1 AP Biology 00762-113-CED-Biology_Unit 3.indd 70 2/28/19 7:21 PM
Lab Manual - Student Edition - OpenStax
Lab 1: Lab Safety, Scientific Method, and Measurements 9 Lab 2: Introduction to Microscopy 16 Lab 3: Macromolecules in Common Food 24 Lab 4: Prokaryotic and Eukaryotic Cells 32 Lab 5: Subcellular Structures 40 Lab 6: Diffusion and Osmosis 50 Lab 7: Factors Affecting the Enzymatic Activity of Lactase 59 Lab 8: Cellular Respiration 68
5.310 (F19) Catalase Kinetics Lab Manual - MIT …
mechanism of enzyme kinetics. The theory proposes that when an enzyme acts upon a substrate molecule, an enzyme-substrate complex forms and that this complex yields the product(s) in addition to the original unchanged enzyme. If the substrate is present in excess over the enzyme, the enzyme would get used up and form enzyme-substrate complexes.
An Inquiry-Based Enzyme Lab - ableweb.org
additional preparation is needed for the inquiry lab. This laboratory series was designed for an introductory biology class but could be adapted for use in more advanced classes as well. The complete lab consists of four modules taught in three 2-hour lab periods. Each lab includes a pre-lab homework assignment, the lab itself, and a post-lab
Looking Into Lactase - Towson University
Enzyme Lab . Maryland Loaner Lab Teacher Packet . Structured Inquiry Version ©Developed by Maryland High School teachers ... Answers 20 . Answers to Part 1 20 . Answers to Part 2 22 . Answers to Part 3 24 . Answers to Brief Constructed Response Questions 26 . Answers to Extension Activities 27 ...
BIOMAN: Enzymatic Game Enzymes and pH Answer the …
17. Sketch the graph of enzyme function and substrate concentration. PICK NEXT CHALLENGE . TAKE THE MINI QUIZ . GO BACK TO THE MAIN MENU . The Enzymatic Machine 18. What is the optimum temperature for the enzyme? 19. What is the optimum pH for the enzyme? 20. Can the enzyme be used more than once? 21. What kind of reaction is being driven by ...
Enzyme Lab: Toothpickase - Weebly
Enzyme Lab: “Toothpickase” (“Toothpickase” is a DIGESTIVE ENZYME. It breaks down toothpicks into two units.) About Enzymes Chemical reactions make life possible. In each individual cell of a human there are many chemical reactions taking place. These chemical reactions involve the breaking and reforming of chemical bonds between
Biotechnology: Restriction Enzyme Analysis of DNA
enzyme recognition palindrome. Thus, an enzyme that recognizes four nucleotides will cut DNA on average once every 256 base pairs, while an enzyme that recognizes fi ve base pairs will cut DNA once every 1024 base pairs. Human chromosomal DNA contains 3 billion base pairs and has a large number of restriction enzyme recognition sites.
AP BIOLOGY LAB #1 - Ely Science Center
ENZYME ACTIVITY This activity is an alternative to the titration proposed for Enzyme Catalysis (AP Bio Lab #2, Biology Lab Manual). There are numerous alternative lab activities that measure the rate of ... results will be submitted along with your graphs and answers to questions. EVERY STUDENT IS RESPONSIBLE FOR RECORDING AND GRAPHING THE
Int esws at Y8 ap sb answers ttpp - Pearson
Answers 8A Food and nutrition 8Aa Nutrients Student Book 1: 8Aa Food and advertising 1 Students’ own answers: e.g. for energy, growth and repair, and health because food contains nutrients, such as proteins and carbohydrates, which are used for different activities in the body match some names of nutrients with their uses in the body
REVIEW QUESTIONS FOR ENZYME KINETICS: ANSWERS …
REVIEW QUESTIONS FOR ENZYME KINETICS: ANSWERS, continued 7. Explain mathmematically how a value for Km can be obtained from the Vo vs So graph when Vo = 1/2 Vmax. When Vo = Vmax/2, then Vmax/2 = Vmax.So , Km + So cancelling Vmax, 1/2 = So or Km + So = 2So Km + So or Km = So at Vo = (value of) Vmax/2 ...
IGCSE Biology (O610) Workbook Chapter 05 Enzymes
• Investigate and describe the effect of changes in temperature and pH on enzyme activity • Investigate and describe the use of biological washing powders that contain enzymes Extended: • Explain enzyme action with reference to the active site, …
35 40 - MRS. HALE'S SCIENCE WEBSITE
This is a “hands-on” lesson in enzyme action, demonstrating the natural increase in reaction rate, the leveling off of the reaction and the subsequent drop in products produced as the substrate ... PRE-LAB QUESTIONS (answer before doing the lab) 1. How can the rate of the reaction be measured? 2. What factors can affect the reaction rate? 3 ...
Low Budget Biology 3: Lactase Enzyme Lab - MRS.
Low Budget Biology 3: Lactase Enzyme Lab Teacher Prep Purpose: this lab will examine the specificity of an enzyme to a specific substrate. Students will observe what happens when the enzyme is denatured. Introduction: Student Information: Lactose, the sugar found in milk, is a disaccharide composed of glucose and galactose (both six sided sugars).
Enzyme Lab Answers (book) - netsec.csuci.edu
your enzyme lab answers, regardless of the specific experiment conducted. We'll dissect common enzyme experiments, explain the key concepts behind the results, and offer tips for troubleshooting any unexpected findings. Get ready to unlock the secrets of your enzyme lab data! Understanding Enzyme Activity: The Basics
INVESTIGATING THE EFFECT OF TEMPERATURE ON ENZYME …
ON ENZYME ACTIVITY Purpose To investigate the effect of temperature on the initial rate of reaction of an enzyme-controlled reaction. To calculate Q10 for an enzyme-controlled reaction. SAFETY Review the students’ risk assessments and discuss any safety considerations. Hydrogen peroxide is an irritant and dangerous if swallowed.
Virtual Lab - Enzyme
4. When an enzyme catalyzes a reaction: a. Substrate(s) bind in the active site b. Products bind in the active site c. The shape of the enzyme remains unchanged d. The enzyme is consumed by the reaction 5. Which of the following would interfere most with the ability of an enzyme to catalyze a reaction? a. Reduced concentration of substrate ...
Enzyme Lab - Doral Academy Preparatory School
Sep 10, 2013 · Enzyme Lab Background information Liver and other living tissues contain the enzyme catalase. This enzyme breaks down hydrogen peroxide, which is a harmful by-product of the process of cellular respiration if it builds up in concentration in the cells. If we use potato or other tissue containing this enzyme, we can use this to measure the relative
AP Biology Toothpickase Lab Name:
Toothpickase Lab Name: _____ PRE-LAB: Please answer the following questions before beginning this lab. 1. Explain this statement: “An enzyme is a catalytic protein”. 2. Explain what is occurring in the diagram on the right. 3. Explain how an enzyme works using the “anatomy of the enzyme” in your explanation.
Name: ENZYMES - Biology by Napier
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TOOTHPICKASE ACTIVITY - ScienceGeek.net
the substrate to be broken down and your hands are an enzyme, complete with an active site (between your fingers and thumb.) Notice that the enzyme (your hand) is much larger than the substrate (toothpicks.) As you will be performing the activity with your eyes closed, this simulates the random contact made between substrate and enzyme.
Catalase Liver Lab - SCISAVVY
In this lab, you will study an enzyme that is found in the cells of many living tissues. The name of the enzyme is catalase (KAT-uh-LAYSS); it speeds up a reaction which breaks down ... Please put your answers into a expository format (write it as if you were telling someone about what you learned, but still maintain 3rd person voice).
Got Lactase? Coevolution of genes and culture film activity …
example, milk is digested by the enzyme lactase, which breaks down lactose (a sugar in milk). • Humans, like all species, evolve and adapt to their environment through natural selection. Lactase persistence is an example of a human adaptation. • For evolution to occur, there must be selection for or against traits. Both the physical and ...
ENZYMES Worksheet 1. 2. b. 2. c. 3. - Denton ISD
a. ____ active site 1. a portion of an enzyme to which a substrate can attach b. ____ anabolism 2. the chemical on which the enzyme acts c. ____ catabolism 3. the result of a chemical reaction d. ____ catalyst 4. a molecule that prevents an enzyme from working e. ____ coenzyme 5. a molecule that makes the active site of an enzyme functional
Amylase: a sample enzyme - umb.edu
Amylase: a sample enzyme Objectives: After completion of this laboratory exercise you will be able to: 1. Explain the importance of enzymes in biology. 2. Explain the basic properties of an enzyme as a catalyst. 3. Discuss the effect of enzyme concentration and various inhibitors on the rate of an enzymatic reaction. 4.
1 E n zy ma ti c Di g e s ti o n o f S ta r c h b y Amy l a s e
enzyme-catalyzed reactions. Specifically, students will alter reaction conditions (such as temperature, pH, and source of amylase) and determine the effects on the rate of ... closed-toe shoes, long pants, and lab coats at all times during this lab activity. Long hair and loose clothing should be pulled/tied back. Hot water, glassware, and ...
Answers - Hodder Education
Possible answers (maximum [3]): • Above 50°C, the enzyme denatures [1] • Increasing temperature increases kinetic energy [1] • Molecules within the enzyme vibrate and bonds holding the enzyme’s shape are broken [1] • The active site changes shape [1] • The substrate does not fit so the enzyme does not break down the substrate [1]
Lambda λ DNA Restriction Digest and Electrophoresis Lab
**In lab book** 2. Centrifuge the tubes for about 3 seconds. Be sure your centrifuge is balanced. **In lab book** 3. Obtain and label 4 micro-test tubes: a. L: for lambda DNA without any added enzymes b. P: for lambda DNA and the restriction enzyme PstI c. E: for lambda DNA and the restriction enzyme EcoRI d.
RESTRICTION ENZYME WORKSHEET #1 - Mrs. Griffin's …
RESTRICTION ENZYME WORKSHEET #1 Name: A natural enemy of bacteria is a virus. To defend when attacked by a virus, bacteria use chemical ... Police lab technicians used the polymerase chain reaction to look at two different chromosqme regions that contain a VNTR. They used one set of primers for each region, The chromosome regions, primers, and ...
Biology corner enzyme lab answers sheets answers pdf
In this lab, you will study an enzyme that is found in the cells of many living tissues. The name of the enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into two harmless substances → water and oxygen. ... Biology corner enzyme lab answers sheets answers pdf ...
AP BIOLOGY 2010 SCORING GUIDELINES - College Board
enzyme. Part (d) instructed students to list two environmental factors that can alter the rate of enzyme-mediated reactions. Students were asked to discuss how each of the two factors they selected would affect the reaction rate. Students were expected to demonstrate an understanding of how environmental variables control an enzyme’s function.
DNA Scissors: Introduction to Restriction Enzymes - Sayre …
restriction enzyme called EcoRI. Its name comes from the bacterium in which it was discovered: Escherichia coli strain RY13 (EcoR), and "I" because it was the first restriction enzyme found in this organism. EcoRI makes one cut between the G and A in each of the DNA strands (see below). After the cuts are made, the
Enzyme Cut-outs Activity - Rancocas Valley Regional High …
Apr 24, 2014 · Enzyme + substrate 1 + substrate 2 enzyme-substrate complex enzyme + product Part D: Graphing Each enzyme works best at a certain temperature and pH. Below or above an enzyme’s optimal temperature or optimal pH, the reaction is slower. 1. Using the data table , graph the data to determine the optimum temperature for
Toothpickase! Enzyme Activity Simulation - Central Bucks …
1. At lower temperatures, an enzyme’s activity will _____. 2. At lower substrate concentrations, an enzyme’s activity will _____. 3. A change in pH will cause an enzyme’s activity to _____. The Simulation: Your hands are an enzyme called “Toothpickase.” Enzymes are typically named after the
AP BIOLOGY Investigation #9 - NJCTL
Aug 16, 2014 · Biotechnology: Restriction Enzyme Analysis of DNA www.njctl.org Summer 2014 Slide 2 / 26 Investigation #9: Restriction Enzyme · Pre-Lab · Guided Investigation · Independent Inquiry C l i c k o n t h e t o p i c t o g o t o t h a t s e c t i o n · Pacing/Teacher's Notes Slide 3 / 26.
Lab 5, Enzymes, Jim 1-18-12 - faculty.umb.edu
Enzymes Learning Goals: After completion of this laboratory exercise you will be able to: 1. Explain the importance of enzymes in biology. 2. Explain the basic properties of an enzyme as a catalyst.
An Inquiry-Based Enzyme Lab - ableweb.org
additional preparation is needed for the inquiry lab. This laboratory series was designed for an introductory biology class but could be adapted for use in more advanced classes as well. The complete lab consists of four modules taught in three 2-hour lab periods. Each lab includes a pre-lab homework assignment, the lab itself, and a post-lab
ENZYME ACTIVITY LAB - Hillsdale College
work. They are specific for their reactions, and are said to fit together like “lock and key.” This lab is examining the effect of temperature on enzyme activity. The liver contains the enzyme catalase, which is responsible for breaking down the substrate hydrogen peroxide (H 2 O 2) in the liver to H 2 O and O 2 gas. H 2 O 2 > H 2 O + O 2
Enzyme Lab - Ms. Abbas AP Biology
in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. * Explain how environmental factors affect the rate of enzyme-catalyzed reactions. Name:_____ Date:_____ Enzyme Lab
LIVER ENZYME LAB The objectives for this lab are for …
LIVER ENZYME LAB The objectives for this lab are for students to: demonstrate the function of an enzyme observe how a change in conditions can affect enzyme function Background: All living cells contain enzymes. One of these enzymes, called catalase, breaks down a substance called hydrogen peroxide (𝐻2𝑂2
