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Control of Gene Expression in Prokaryotes: A POGIL Deep Dive
Introduction:
Unraveling the intricate mechanisms of gene expression is fundamental to understanding the life of all organisms. Prokaryotes, the simplest life forms, offer a surprisingly sophisticated, yet accessible, model for studying this process. This post dives deep into the control of gene expression in prokaryotes, leveraging the POGIL (Process Oriented Guided Inquiry Learning) approach to guide you through the key concepts and mechanisms. We'll explore operons, transcription factors, and environmental influences, all within the context of a highly engaging learning experience. Prepare to master the intricacies of prokaryotic gene regulation!
1. Understanding the Basics: What is Gene Expression?
Before delving into the specifics of prokaryotic control, let's establish a foundational understanding. Gene expression is the process by which information encoded in a gene is used to synthesize a functional gene product, typically a protein. This involves two major steps: transcription (DNA to RNA) and translation (RNA to protein). In prokaryotes, these processes often occur concurrently in the cytoplasm, unlike eukaryotes where they are spatially separated.
2. The Operon Model: A Core Concept in Prokaryotic Gene Regulation
The operon is a central feature of prokaryotic gene expression control. It's a cluster of genes transcribed together under the control of a single promoter. The lac operon, often used as a quintessential example, regulates the metabolism of lactose in E. coli.
#### 2.1 The Components of the lac Operon:
Promoter: The region where RNA polymerase binds to initiate transcription.
Operator: A short DNA sequence that acts as a switch, binding repressor proteins to prevent transcription.
Structural Genes: The genes encoding proteins involved in lactose metabolism (e.g., β-galactosidase).
Repressor Protein: A protein that binds to the operator, blocking RNA polymerase and preventing transcription.
Inducer (Lactose): A molecule that binds to the repressor protein, causing a conformational change that prevents it from binding to the operator, thus allowing transcription.
#### 2.2 The lac Operon in Action:
When lactose is absent, the repressor protein binds to the operator, blocking transcription. However, when lactose is present, it acts as an inducer, binding to the repressor, changing its shape, and preventing it from binding the operator. This allows RNA polymerase to transcribe the structural genes, producing the enzymes needed for lactose metabolism.
3. Beyond the lac Operon: Other Regulatory Mechanisms
The lac operon exemplifies inducible operons, where transcription is activated by the presence of a specific molecule. However, other operons function differently, such as repressible operons, where transcription is turned off by the presence of a molecule. These variations showcase the versatility and sophistication of prokaryotic gene regulation.
#### 3.1 Repressible Operons (e.g., trp operon):
These operons are typically involved in the biosynthesis of amino acids. Transcription is normally on but is repressed by the presence of the end product (e.g., tryptophan). The end product acts as a corepressor, binding to a repressor protein, allowing it to bind to the operator and block transcription.
#### 3.2 Positive Regulation:
Some operons are controlled by activator proteins that enhance RNA polymerase binding to the promoter, increasing transcription rates. These activators often respond to specific environmental signals, allowing the cell to fine-tune gene expression based on its needs.
4. Environmental Influences on Gene Expression:
Prokaryotic gene expression is highly responsive to environmental cues. Changes in nutrient availability, temperature, or other environmental stressors can trigger changes in gene expression through various mechanisms, including:
Two-component regulatory systems: These systems involve a sensor kinase that detects environmental changes and a response regulator that alters gene expression accordingly.
Quorum sensing: This mechanism allows bacteria to coordinate gene expression based on population density.
5. Connecting POGIL to Prokaryotic Gene Expression:
The POGIL approach emphasizes active learning and critical thinking. Applying this method to understanding prokaryotic gene expression involves working through problem-solving activities, analyzing experimental data, and developing a deeper understanding of the underlying mechanisms. By engaging with POGIL activities centered on operons and regulatory elements, students can develop a robust understanding of this critical area of biology.
Conclusion:
Control of gene expression in prokaryotes is a remarkable feat of biological engineering, enabling these organisms to adapt and thrive in diverse environments. The operon model, along with various other regulatory mechanisms, provides a finely tuned system for controlling gene activity, ensuring efficient resource utilization and optimal cellular function. By employing active learning strategies like POGIL, students can gain a comprehensive and lasting understanding of this fascinating biological process.
FAQs:
1. What is the difference between inducible and repressible operons? Inducible operons are switched "on" by the presence of a specific molecule (inducer), while repressible operons are switched "off" by the presence of a specific molecule (corepressor).
2. How does the environment influence prokaryotic gene expression? Environmental factors such as nutrient availability, temperature, and population density can trigger signal transduction pathways, leading to changes in the expression of specific genes.
3. What are some examples of positive regulation in prokaryotes? The activation of the lac operon by CAP protein in the presence of cAMP is a classic example of positive regulation.
4. How does quorum sensing work? Quorum sensing involves the production and detection of signaling molecules called autoinducers. When the concentration of autoinducers reaches a threshold, it triggers changes in gene expression that are coordinated across the bacterial population.
5. How can POGIL activities enhance understanding of prokaryotic gene regulation? POGIL activities promote collaborative learning and problem-solving, allowing students to actively construct their understanding of complex concepts rather than passively receiving information. This deeper engagement leads to better retention and a more robust understanding of prokaryotic gene regulation.
control of gene expression in prokaryotes pogil: Biology for AP ® Courses Julianne Zedalis, John Eggebrecht, 2017-10-16 Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences. |
control of gene expression in prokaryotes pogil: Interaction of Translational and Transcriptional Controls in the Regulation of Gene Expression Marianne Grunberg-Manago, 2012-12-02 Interaction of Translational and Transcriptional Controls in the Regulation of Gene Expression presents the proceedings of the Fogarty International Conference on Translational/Transcriptional Regulation of Gene Expression, held at the National Institutes of Health in Bethesda, Maryland, on April 7-9, 1982. Speakers discussed the molecular strategies at work during the modulation of gene expression following transcriptional initiation. They also discussed recent developments in a number of key areas in which transcriptional and translational components interact. Organized into five sections encompassing 36 chapters, this volume explores both prokaryotic and eukaryotic systems, as well as structure-function correlations. It begins with an overview of translational/transcriptional controls in prokaryotes, the regulation of gene expression by transcription termination and RNA processing, and the structure and expression of initiation factor genes. It then examines the effect of the codon context on translational fidelity, including mistranslation of messenger RNA; protein synthesis for the construction of cell architecture; regulation of initiation factor activity; and translational regulation in cells. This book is a valuable resource for Fogarty International Scholars who want to broaden their knowledge and contribute their expertise to the National Institutes of Health community. |
control of gene expression in prokaryotes pogil: Principles of Biology Lisa Bartee, Walter Shiner, Catherine Creech, 2017 The Principles of Biology sequence (BI 211, 212 and 213) introduces biology as a scientific discipline for students planning to major in biology and other science disciplines. Laboratories and classroom activities introduce techniques used to study biological processes and provide opportunities for students to develop their ability to conduct research. |
control of gene expression in prokaryotes pogil: The Operon Jeffrey H. Miller, William S. Reznikoff, 1980 |
control of gene expression in prokaryotes pogil: Control of Gene Expression Norman Maclean, 1976 The control of gene expression and its levels of action; Gene expression in prokaryotes; Experimental systems of differential gene fuction in eukaryotes-systems involving one type of protein; Experimental systems of differential gene fuction in eukaryotes-systems of limited complexity; Experimental systems of differential gene fuction in eukaryotes-systems not well understood in molecular terms; RNA involvement in gene expression; General concepts of gene regulation. |
control of gene expression in prokaryotes pogil: Eukaryotic Gene Expression Ajit Kumar, 2013-03-09 The recent surge of interest in recombinant DNA research is understandable considering that biologists from all disciplines, using recently developed mo lecular techniques, can now study with great precision the structure and regulation of specific genes. As a discipline, molecular biology is no longer a mere subspeciality of biology or biochemistry: it is the new biology. Current approaches to the outstanding problems in virtually all the traditional disci plines in biology are now being explored using the recombinant DNA tech nology. In this atmosphere of rapid progress, the role of information exchange and swift publication becomes quite crucial. Consequently, there has been an equally rapid proliferation of symposia volumes and review articles, apart from the explosion in popular science magazines and news media, which are always ready to simplify and sensationalize the implications of recent dis coveries, often before the scientific community has had the opportunity to fully scrutinize the developments. Since many of the recent findings in this field have practical implications, quite often the symposia in molecular biology are sponsored by private industry and are of specialized interest and in any case quite expensive for students to participate in. Given that George Wash ington University is a teaching institution, our aim in sponsoring these Annual Spring Symposia is to provide, at cost, a forum for students and experts to discuss the latest developments in selected areas of great significance in biology. Additionally, since the University is located in Washington, D. C. |
control of gene expression in prokaryotes pogil: Preparing for the Biology AP Exam Neil A. Campbell, Jane B. Reece, Fred W. Holtzclaw, Theresa Knapp Holtzclaw, 2009-11-03 Fred and Theresa Holtzclaw bring over 40 years of AP Biology teaching experience to this student manual. Drawing on their rich experience as readers and faculty consultants to the College Board and their participation on the AP Test Development Committee, the Holtzclaws have designed their resource to help your students prepare for the AP Exam. Completely revised to match the new 8th edition of Biology by Campbell and Reece. New Must Know sections in each chapter focus student attention on major concepts. Study tips, information organization ideas and misconception warnings are interwoven throughout. New section reviewing the 12 required AP labs. Sample practice exams. The secret to success on the AP Biology exam is to understand what you must know and these experienced AP teachers will guide your students toward top scores! |
control of gene expression in prokaryotes pogil: Basic Concepts in Biochemistry: A Student's Survival Guide Hiram F. Gilbert, 2000 Basic Concepts in Biochemistry has just one goal: to review the toughest concepts in biochemistry in an accessible format so your understanding is through and complete.--BOOK JACKET. |
control of gene expression in prokaryotes pogil: The Making of the Fittest: DNA and the Ultimate Forensic Record of Evolution Sean B. Carroll, 2007-08-28 A geneticist discusses the role of DNA in the evolution of life on Earth, explaining how an analysis of DNA reveals a complete record of the events that have shaped each species and how it provides evidence of the validity of the theory of evolution. |
control of gene expression in prokaryotes pogil: Control of Messenger RNA Stability Joel Belasco, Joel G. Belasco, George Brawerman, 1993-04-06 This is the first comprehensive review of mRNA stability and its implications for regulation of gene expression. Written by experts in the field, Control of Messenger RNA Stability serves both as a reference for specialists in regulation of mRNA stability and as a general introduction for a broader community of scientists. Provides perspectives from both prokaryotic and eukaryotic systems Offers a timely, comprehensive review of mRNA degradation, its regulation, and its significance in the control of gene expression Discusses the mechanisms, RNA structural determinants, and cellular factors that control mRNA degradation Evaluates experimental procedures for studying mRNA degradation |
control of gene expression in prokaryotes pogil: POGIL Activities for AP Biology , 2012-10 |
control of gene expression in prokaryotes pogil: Prokaryotic Gene Expression Simon Baumberg, 1999-05-27 Prokaryotic gene expression is not only of theoretical interest but also of highly practical significance. It has implications for other biological problems, such as developmental biology and cancer, brings insights into genetic engineering and expression systems, and has consequences for important aspects of applied research. For example, the molecular basis of bacterial pathogenicity has implications for new antibiotics and in crop development. Prokaryotic Gene Expression is a major review of the subject, providing up-to-date coverage as well as numerous insights by the prestigious authors. Topics covered include operons; protein recognition of sequence specific DNA- and RNA-binding sites; promoters; sigma factors, and variant tRNA polymerases; repressors and activators; post-transcriptional control and attenuation; ribonuclease activity, mRNA stability, and translational repression; prokaryotic DNA topology, topoisomerases, and gene expression; regulatory networks, regulatory cascades and signal transduction; phosphotransfer reactions; switch systems, transcriptional and translational modulation, methylation, and recombination mechanisms; pathogenicity, toxin regulation and virulence determinants; sporulation and genetic regulation of antibiotic production; origins of regulatory molecules, selective pressures and evolution of prokaryotic regulatory mechanisms systems. Over 1100 references to the primary literature are cited. Prokaryotic Gene Expression is a comprehensive and authoritative review of current knowledge and research in the area. It is essential reading for postgraduates and researchers in the field. Advanced undergraduates in biochemistry, molecular biology, and microbiology will also find this book useful. |
control of gene expression in prokaryotes pogil: Gene Regulation in Eukaryotes Edgar Wingender, 1993 A much-needed guide through the overwhelming amount of literature in the field. Comprehensive and detailed, this book combines background information with the most recentinsights. It introduces current concepts, emphasizing the transcriptional control of genetic information. Moreover, it links data on the structure of regulatory proteins with basic cellular processes. Both advanced students and experts will find answers to such intriguing questions as: - How are programs of specific gene repertoires activated and controlled? - Which genes drive and control morphogenesis? - Which genes govern tissue-specific tasks? - How do hormones control gene expression in coordinating the activities of different tissues? An abundant number of clearly presented glossary terms facilitates understanding of the biological background. Speacial feature: over 2200 (!) literature references. |
control of gene expression in prokaryotes pogil: Molecular Biology of the Cell , 2002 |
control of gene expression in prokaryotes pogil: The Pancreatic Beta Cell , 2014-02-20 First published in 1943, Vitamins and Hormones is the longest-running serial published by Academic Press. The Series provides up-to-date information on vitamin and hormone research spanning data from molecular biology to the clinic. A volume can focus on a single molecule or on a disease that is related to vitamins or hormones. A hormone is interpreted broadly so that related substances, such as transmitters, cytokines, growth factors and others can be reviewed. This volume focuses on the pancreatic beta cell. - Expertise of the contributors - Coverage of a vast array of subjects - In depth current information at the molecular to the clinical levels - Three-dimensional structures in color - Elaborate signaling pathways |
control of gene expression in prokaryotes pogil: Biophysical Chemistry James P. Allen, 2009-01-26 Biophysical Chemistry is an outstanding book that delivers both fundamental and complex biophysical principles, along with an excellent overview of the current biophysical research areas, in a manner that makes it accessible for mathematically and non-mathematically inclined readers. (Journal of Chemical Biology, February 2009) This text presents physical chemistry through the use of biological and biochemical topics, examples and applications to biochemistry. It lays out the necessary calculus in a step by step fashion for students who are less mathematically inclined, leading them through fundamental concepts, such as a quantum mechanical description of the hydrogen atom rather than simply stating outcomes. Techniques are presented with an emphasis on learning by analyzing real data. Presents physical chemistry through the use of biological and biochemical topics, examples and applications to biochemistry Lays out the necessary calculus in a step by step fashion for students who are less mathematically inclined Presents techniques with an emphasis on learning by analyzing real data Features qualitative and quantitative problems at the end of each chapter All art available for download online and on CD-ROM |
control of gene expression in prokaryotes pogil: The Double Helix James D. Watson, 1969-02 Since its publication in 1968, The Double Helix has given countless readers a rare and exciting look at one highly significant piece of scientific research-Watson and Crick's race to discover the molecular structure of DNA. |
control of gene expression in prokaryotes pogil: Translational Control of Gene Expression Nahum Sonenberg, John W. B. Hershey, Michael B. Mathews, 2001 Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control. |
control of gene expression in prokaryotes pogil: The Molecular Basis of Heredity A.R. Peacocke, R.B. Drysdale, 2013-12-17 |
control of gene expression in prokaryotes pogil: Concepts of Biology Samantha Fowler, Rebecca Roush, James Wise, 2023-05-12 Black & white print. Concepts of Biology is designed for the typical introductory biology course for nonmajors, covering standard scope and sequence requirements. The text includes interesting applications and conveys the major themes of biology, with content that is meaningful and easy to understand. The book is designed to demonstrate biology concepts and to promote scientific literacy. |
control of gene expression in prokaryotes pogil: Regulation of Gene Expression Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters, 2008-08-17 The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell. |
control of gene expression in prokaryotes pogil: Transcription Regulation in Prokaryotes Rolf Wagner, 2000 'I therefore regard this book as a standard, extremely suitable not only for teaching to 3rd or 4th year undergraduate students with interest in cellular biology and molecular microbiology, but also for senior scientists who have research interests in prokaryotic transcription regulation2 Cell Biology International'a superb, compact yet comprehensive, treatise on the regulation of gene expression, principally but not exclusively, in E.Coli and its phage... A must for all students at undergraduate or postgraduate level and also for reseachers of eukaryotic transcription who need reminding of a few paradigms' AslibThis text is written for advanced students with a basic background in molecular biology and provides a clear and concise summary of the flow of information from genes to proteins in simple prokaryotic cells. Transcription regulation is of central importance to molecular biology, and in bacterial cells the major regulatory stage is transcription. While most textbooks cover transcription in a single chapter with a strong emphasis on eukaryotic transcription, this new text is devoted to prokaryotic transcription and is perfect for use on molecular biology, microbiology and technology courses. |
control of gene expression in prokaryotes pogil: Post-transcriptional Control of Gene Expression Orna Resnekov, Alexander von Gabain, 2013-06-29 Many important cellular processes rely on posttranscriptional control of gene expression. This book describes the mechanisms of gene expression at this level that occur in the cytoplasm of prokaryotes and eukaryotes. Several introductory chapters discuss the general principles of translation and mRNA stability. The interactions of mature mRNA with the translational machinery, the components of mRNA degradation and antisense RNA are surveyed. Subsequent chapters discuss protein folding, transport, modification and degradation. The book is an invaluable source of information for both newcomers and those wishing an overview of the field. |
control of gene expression in prokaryotes pogil: Anatomy of Gene Regulation Panagiotis A. Tsonis, 2003-01-13 No longer simple line drawings on a page, molecular structures can now be viewed in full-figured glory, often in color and even with interactive possibilities. Anatomy of Gene Regulation is the first book to present the parts and processes of gene regulation at the three-dimensional level. Vivid structures of nucleic acids and their companion proteins are revealed in full-color, three-dimensional form. Beginning with a general introduction to three-dimensional structures, the book looks at the organization of the genome, the structure of DNA, DNA replication and transcription, splicing, protein synthesis, and ultimate protein death. Throughout, the text employs a discussion of genetics and structural mechanics. The concise and unique synthesis of information will offer insight into gene regulation, and into the development of methods to interfere with regulation at diseased states. This textbook and its accompanying web site are appropriate for both undergraduate and graduate students in genetics, molecular biology, structural biology, and biochemistry courses. |
control of gene expression in prokaryotes pogil: Uncovering Student Ideas in Science: 25 formative assessment probes Page Keeley, 2005 V. 1. Physical science assessment probes -- Life, Earth, and space science assessment probes. |
control of gene expression in prokaryotes pogil: The Na, K-ATPase Jean-Daniel Horisberger, 1994 This text addresses the question, How does the sodium pump pump'. A variety of primary structure information is available, and progress has been made in the functional characterization of the Na, K-pump, making the answer to this question possible, within reach of currently used techniques |
control of gene expression in prokaryotes pogil: Biological Regulation and Development Robert Goldberger, 2012-12-06 The motivation for us to produce a treatise on regulation was mainly our convic tion that it would be fun, and at the same time productive, to approach the subject in a way that differs from that of other treatises. We had ourselves written reviews for various volumes over the years, most of them bringing together all possible facts relevant to a particular operon, virus, or biosynthetic system. And we were not convinced of the value of such reviews for anyone but the expert in the field reviewed. We thought it might be more interesting and more instructive-for both author and reader-to avoid reviewing topics that anyone scientist might work on, but instead to review the various parts of what many different scientists work on. Cutting across the traditional boundaries that have separated the subjects in past volumes on regulation is not an easy thing to do-not because it is difficult to think of what interesting topics should replace the old ones, but because it is difficult to find authors who possess sufficient breadth of knowledge and who are willing to write about areas outside those pursued in their own laboratories. For example, no one scientist works on suppression per se. He may study the structure of suppressor tRNAs in Escherichia coli, he may study phenotypic suppression of various characters in drosophila, he may study polarity in gene expression, and so on. |
control of gene expression in prokaryotes pogil: Basics of Foundation Design Bengt Fellenius, 2017-03-17 The Red Book presents a background to conventional foundation analysis and design. The text is not intended to replace the much more comprehensive 'standard' textbooks, but rather to support and augment these in a few important areas, supplying methods applicable to practical cases handled daily by practising engineers and providing the basic soil mechanics background to those methods. It concentrates on the static design for stationary foundation conditions. Although the topic is far from exhaustively treated, it does intend to present most of the basic material needed for a practising engineer involved in routine geotechnical design, as well as provide the tools for an engineering student to approach and solve common geotechnical design problems. |
control of gene expression in prokaryotes pogil: Geometric and Ergodic Aspects of Group Actions S. G. Dani, Anish Ghosh, 2020-01-13 This book gathers papers on recent advances in the ergodic theory of group actions on homogeneous spaces and on geometrically finite hyperbolic manifolds presented at the workshop “Geometric and Ergodic Aspects of Group Actions,” organized by the Tata Institute of Fundamental Research, Mumbai, India, in 2018. Written by eminent scientists, and providing clear, detailed accounts of various topics at the interface of ergodic theory, the theory of homogeneous dynamics, and the geometry of hyperbolic surfaces, the book is a valuable resource for researchers and advanced graduate students in mathematics. |
control of gene expression in prokaryotes pogil: Post-Transcriptional Control of Gene Expression in Plants Witold Filipowicz, Thomas Hohn, 2012-12-06 A recent volume of this series (Signals and Signal Transduction Pathways in Plants (K. Palme, ed.) Plant Molecular Biology 26, 1237-1679) described the relay races by which signals are transported in plants from the sites of stimuli to the gene expression machinery of the cell. Part of this machinery, the transcription apparatus, has been well studied in the last two decades, and many important mechanisms controlling gene expression at the transcriptional level have been elucidated. However, control of gene expression is by no means complete once the RNA has been produced. Important regulatory devices determine the maturation and usage of mRNA and the fate of its translation product. Post-transcriptional regulation is especially important for generating a fast response to environmental and intracellular signals. This book summarizes recent progress in the area of post-transcriptional regulation of gene expression in plants. 18 chapters of the book address problems of RNA processing and stability, regulation of translation, protein folding and degradation, as well as intracellular and cell-to-cell transport of proteins and nucleic acids. Several chapters are devoted to the processes taking place in plant organelles. |
control of gene expression in prokaryotes pogil: Molecular Mechanisms in the Control of Gene Expression Donald P. Nierlich, W.J. Rutter, C. Fred Fox, 2013-10-22 Molecular Mechanisms in the Control of Gene Expression documents the proceedings of the ICN-UCLA conference on Molecular Mechanisms in the Control of Gene Expression, organized through the Molecular Biology Institute of UCLA, held in Keystone, Colorado, 21-26 March 1976. The conference focused on three topics: the action of repressors on specific nucleotide sequences in DNA; how DNA and histones are intertwined in eucaryotic chromosomes; and in the development of new techniques that appear to lift genes from complex genomes. The volume contains 65 chapters organized into nine parts. The papers in Part I examine the organization of prokaryotic and eukaryotic chromosomes. Part II presents studies on the interaction of RNA a polymerase and regulatory molecules with defined DNA sites. Parts III and IV focus on RNA polymerases of eukaryotes and the regulation of transcription in eukaryotic systems, respectively. Part V contains papers dealing with nucleic acid sequences, transcription, and processing. Part VI covers cellular aspects in the study of gene expression. Part VII takes up cloning while Part VIII is devoted to genetic analysis through restriction mapping and molecular cloning. Finally, Part IX summarizes the recent progress reported at the conference and also indicates some of the limitations that can be placed upon interpretation of data. |
control of gene expression in prokaryotes pogil: Signal Transduction in Plants P. Aducci, 1997 The molecular aspects of recognition and transduction of different kinds of signals is a research area that is spawning increasing interest world-wide. Major advances have been made in animal systems but recently plants too, have become particularly attractive because of their promising role in biotechnology. The type of signals peculiar to the plant world and the similarity of plant transduction pathways investigated thus far to their animal counterparts are prompting more and more studies in this modern area of cell biology. The present book provides a comprehensive survey of all aspects of the recognition and transduction of plant signals of both chemical and physical origin such as hormones, light, toxins and elicitors. The contributing authors are drawn from diverse areas of plant physiology and plant molecular biology and present here different approaches to studying the recognition and transduction of different signals which specifically trigger molecular processes in plants. Recent advances in the field are reviewed, providing the reader with the current state of knowledge as well as insight into research perspectives and future developments. The book should interest a wide audience that includes not only researchers, advanced students, and teachers of plant biology, biochemistry and agriculture, but it has also significant implications for people working in related fields of animal systems. |
control of gene expression in prokaryotes pogil: Gene Regulation : A Eukaryotic Perspective David S. Latchman, 1990-05-24 |
control of gene expression in prokaryotes pogil: Study Guide 1 DCCCD Staff, Dcccd, 1995-11 |
control of gene expression in prokaryotes pogil: Translational Regulation of Gene Expression 2 J. Ilan, 2012-12-06 This book, which results from the dramatic increase in interest in the control mechanism employed in gene expression and the importance of the regulated proteins, presents new information not covered in Translational Regulation of Gene Expression, which was published in 1987. It is not a revision of the earlier book but, rather, an extension of that volume witl, special emphasis on mecha nIsm. As the reader will discover, there is enormous diversity in the systems employing genes for translational regulation in order to regulate the appearance of the final product-the protein. Thus, we find that important proteins such as protooncogenes, growth factors, stress proteins, cytokines, lymphokines, iron storage and iron-uptake proteins, and a panorama of prokaryotic proteins, as well as eukaryotic viral proteins, are translationally regulated. Since for some gene products the degree of control is greater by a few orders of magnitude than their transcription, we can state that for these genes, at least, the expression is translationall y controlled. Translational regulation of gene expression in eukaryotes has emerged in the last few years as a major research field. The present book describes mechanisms of translational regulation in bacteria, yeast, and eukaryotic viruses, as well as in eukaryotic genes. In this book we try to provide in-depth coverage by including important examples from each group rather than systematically including all additional systems not described in the previous volume. |
control of gene expression in prokaryotes pogil: Hormonal Control of Reproduction Colin Russell Austin, Roger Valentine Short, 1984 In this, our Second Edition of Reproduction in Mammals, we are responding to numerous requests for a more up-to-date and rather more detailed treatment of the subject. The First Edition was accorded an excellent reception, but the first five books were written ten years ago and inevitably there have been advances on many fronts since then. As before, the manner of presentation is intended to make the subject matter interesting to read and readily comprehensible to undergraduates in the biological sciences, and yet with sufficient depth to provide a valued source of information to graduates engaged in both teaching and research. Our authors have been selected from among the best known in their respective fields. This volume discusses the manifold ways in which hormones control the reproductive processes in male and female mammals. The hypothalamus regulates both the anterior and posterior pituitary glands, whilst the pineal can exert a modulating influence on the hypothalamus. The pituitary gonadotrophins regulate the endocrine and gametogenic activities of the gonads, and there are important local feedback effects of hormones within the gonads themselves. Non-pregnant females display many different types of oestrous or menstrual cycles, and there are likewise great species differences in the endocrinology of pregnancy. But the hallmark of mammals is lactation, and this also exerts a major control on subsequent reproductive activity. |
control of gene expression in prokaryotes pogil: Medical Microbiology Illustrated S. H. Gillespie, 2014-06-28 Medical Microbiology Illustrated presents a detailed description of epidemiology, and the biology of micro-organisms. It discusses the pathogenicity and virulence of microbial agents. It addresses the intrinsic susceptibility or immunity to antimicrobial agents. Some of the topics covered in the book are the types of gram-positive cocci; diverse group of aerobic gram-positive bacilli; classification and clinical importance of erysipelothrix rhusiopathiae; pathogenesis of mycobacterial infection; classification of parasitic infections which manifest with fever; collection of blood for culture and control of substances hazardous to health. The classification and clinical importance of neisseriaceae is fully covered. The definition and pathogenicity of haemophilus are discussed in detail. The text describes in depth the classification and clinical importance of spiral bacteria. The isolation and identification of fungi are completely presented. A chapter is devoted to the laboratory and serological diagnosis of systemic fungal infections. The book can provide useful information to microbiologists, physicians, laboratory scientists, students, and researchers. |
control of gene expression in prokaryotes pogil: Evolution of Metabolic Pathways R. Ibrahim, L. Varin, V. De Luca, John Romeo, 2000-09-15 The past decade has seen major advances in the cloning of genes encoding enzymes of plant secondary metabolism. This has been further enhanced by the recent project on the sequencing of the Arabidopsis genome. These developments provide the molecular genetic basis to address the question of the Evolution of Metabolic Pathways. This volume provides in-depth reviews of our current knowledge on the evolutionary origin of plant secondary metabolites and the enzymes involved in their biosynthesis. The chapters cover five major topics: 1. Role of secondary metabolites in evolution; 2. Evolutionary origins of polyketides and terpenes; 3. Roles of oxidative reactions in the evolution of secondary metabolism; 4. Evolutionary origin of substitution reactions: acylation, glycosylation and methylation; and 5. Biochemistry and molecular biology of brassinosteroids. |
control of gene expression in prokaryotes pogil: DNA National Science Foundation (U.S.), 1983 Essays discuss recombinant DNA research, and the structure, mobility, and self-repairing mechanisms of DNA. |
control of gene expression in prokaryotes pogil: Plant Organelles Eric Reid, 1979 |
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